Identify the best potential drug candidates

The challenge

Characterising novel therapeutics during hit-to-lead, lead optimisation and candidate selection can be challenging:

  • A lack of access to the right equipment and assays impact the quality of your data
  • Training internal staff on specific assays requires time, money and resources and limits the number and range of assays you can effectively perform
  • The pressures imposed by ambitious milestones and finite budgets mean you sacrifice data depth and thereby lose important insights

The Arctoris approach to compound characterisation

Our end-to-end automated compound characterisation generates deeper and more robust data – in a fraction of the time normally required. This allows you to benefit from precise, reliable and reproducible results throughout the drug discovery process. By partnering with our team, you will be able to select the best possible drug candidates faster and with improved confidence.

The benefits of our end-to-end, automated cell-based and molecular-biology assays include:

Deep compound profiling and characterisation

Collect a rich dataset with all assays run to generate multidimensional data packages that measure every relevant parameter.

Our automated approach ensures every experiment is conducted the same way every single time, generating deep, reproducible data you can rely on.

Flexible access to a broad range of assays

Gain access to a wide range of assays, so you do not have need to invest in your own lab infrastructure - variable instead of fixed costs

By partnering with our senior team of experienced drug discovery experts, you will be able to select the best possible drug candidates faster and with absolute confidence.

Speed, accuracy and reproducibility

Generate data of unprecedented robustness and reproducibility via our fully automated process, eliminating human error & variability

Enjoy vastly reduced cycle times, helping you to scale your operations and make crucial go/no-go decisions as soon as possible.

Cell-based and molecular biology assays

We offer a broad range of cell-based assays to evaluate the effects of novel therapeutic agents such as small molecules, antibodies, antisense oligonucleotides, and peptides. Our platform enables target identification and validation, antibody and compound screening, and the generation of mechanistic insights. Our key molecular biology assays support you in elucidating patterns and changes in gene and protein expression. All assays are performed on quality-controlled cell lines with audited provenance and guaranteed authenticity and with highest quality research reagents.  

Cell viability

Monitor cell health using a range of viability markers
  • ATP level measurement
  • Reducing potential measurement
  • Live cell protease activity
  • Proliferation measurement & cell cycle analysis
  • Colony formation assays

Cytotoxicity

Assess cell death using distinct markers
  • Lipid bilayer disruption and/ or DNA exposure
  • Caspase activity measurement
  • Necrosis vs apoptosis assessments
  • γH2AX foci for DNA damage response assessment
  • Mitochondrial membrane potential disruption

Multiplex assays

Combine assays to gain more data from the same plate
  • Viability and cytotoxicity
  • Viability and apoptosis
  • Viability, necrosis and apoptosis
  • Viability and metabolic assays
  • Viability and HDAC enzyme activity

Pathway activity analysis

In-cell assays to determine changes in pathways and systems
  • Proteasome activity measurement
  • HDAC enzyme activity measurement
  • NF-κB activity
  • PI3K/ AKT/ mTOR pathway activity
  • JAK/ STAT pathway activity

Metabolic health assays

In-cell assays to determine changes in metabolism
  • Glucose uptake
  • ADP/ ATP level measurement & cAMP production
  • Mitochondrial function & ROS measurement
  • NAD(P)+/ NADPH level measurement
  • Autophagy

Target engagement assays

NanoBRET™ technology to assess drug binding
  • Epigenetic regulators (incl. HDACs & BETs)
  • Transcription factors (incl. cMYC, ER, FOXP3)
  • A wide range of kinases (incl. MEK/ ERK, KRAS)
  • Membrane receptors (incl. EGFR, IL17R, MET-GRB2)
  • Custom design for your proteins of interest

Protein level quantification

Measure levels of your protein of interest
  • Cytokine detection via ELISA or Lumit™
  • Total protein quantitation via Lumit™
  • Post-translational modification via Lumit™
  • Protein localisation via immunofluorescence
  • Immunoblotting/ Western blotting

Gene expression measurement

Confirm changes in gene expression across your targets
  • qRT-PCR and digital PCR
  • Gene expression profiling for a range of gene panels
  • TempO-Seq™ for genome-wide panels
  • Sequencing (RNA Seq, DNA Seq, miRNA Seq)
  • Transcriptional response assessment

3D co-culture and imaging

Additional cell-based research techniques
  • Live cell imaging
  • Cell morphology assessment
  • Phenotypic screens
  • Organoids and spheroids
  • Co-culture cell systems

READY TO GET STARTED?

To find out how to accelerate your project timelines and generate higher quality data, reach out to us today.