Drug-induced liver injury (DILI) remains a leading cause of drug attrition and post-market withdrawal, underscoring the need for more predictive in vitro toxicity models. While primary human hepatocytes (PHH) offer strong physiological relevance, conventional hepatotoxicity assays often rely on single-endpoint readouts that fail to capture temporal dynamics and complex cellular responses, limiting mechanistic insight and predictive power.
This poster presents a multiplexed, high-content approach integrating real-time viability kinetics with Cell Painting phenotypic profiling in PHH. Enabled by Arctoris’ Ulysses® automation platform, the workflow combines precise cell handling, kinetic measurements and high-content imaging to deliver scalable, reproducible, and information-rich toxicity assessments. This approach allows simultaneous capture of functional and morphological responses, improving sensitivity and biological interpretation for predictive toxicology.
Download the poster to discover:
- How multiplexed Cell Painting and viability kinetics improve sensitivity and mechanistic insight over traditional single-endpoint assays
- How Ulysses® enables scalable automation of high-content hepatotoxicity workflows in physiologically relevant primary human hepatocytes
- How integration of time-resolved viability data enhances understanding of compound-induced toxicity dynamics
- Evidence of robust, reproducible phenotypic profiling across compounds and conditions in PHH
- How the platform supports improved toxicity prediction and translational decision-making in drug discovery